| Sat 02/08/25 | Trafford v Barnoldswick Town (CUP) | 2-1 | | | | | | |
| Sat 16/08/25 | Euxton Villa v Barnoldswick Town | 2-3 | | | | | | |
| Mon 25/08/25 | Barnoldswick Town v Prestwich Heys | 2-2 | | | |  | | |
| Sat 30/08/25 | Pilkington v Barnoldswick Town | 3-3 | | | | | | |
| Sat 06/09/25 | Wythenshawe v Barnoldswick Town | 3-0 | | | | | | |
| Sat 13/09/25 | West Didsbury & Chorlton v Barnoldswick Town | 4-0 | | | | | | |
| Tue 16/09/25 | Longridge Town v Barnoldswick Town | 1-2 | | | | | | |
| Sat 20/09/25 | Barnoldswick Town v Sunderland West End (CUP) | 10-1 |  | | | | | |
| Tue 23/09/25 | Barnoldswick Town v Stockport Town | 3-3 | | | | | | |
| Sat 27/09/25 | Longridge Town v Barnoldswick Town (CUP) | 1-3 | | | | | | |
| Sat 11/10/25 | AFC Blackpool v Barnoldswick Town (CUP) | 3-0 | | | | | | |
| Sat 17/01/26 | Barnoldswick Town v AFC Liverpool * | 0-7 | | | | | | |
| Sat 31/01/26 | Barnoldswick Town v Litherland REMYCA * | 2-2 | | | | | | |
| Tue 03/02/26 | Barnoldswick Town v Longridge Town * | 2-5 | | | | | | |
| Sat 07/02/26 | Abbey Hey v Barnoldswick Town | 1-1 | | | | | | |
| Tue 10/02/26 | Barnoldswick Town v Irlam | 0-3 | | | | | | |
| Tue 17/02/26 | Barnoldswick Town v Atherton LR | 1-3 | | | | | | |
| Sat 21/02/26 | South Liverpool v Barnoldswick Town | 3-1 | | | | | | |
| Tue 24/02/26 | Barnoldswick Town v Abbey Hey | 2-0 | |  | | | | |
| Tue 03/03/26 | Glossop North End v Barnoldswick Town | 1-1 | | | | | | |
| Sat 07/03/26 | AFC Liverpool v Barnoldswick Town | 3-1 | | | | | |  |
| Tue 10/03/26 | Charnock Richard v Barnoldswick Town | 3-1 | | | | | | |
| Sat 14/03/26 | Barnoldswick Town v Cheadle Town | 3-1 | | | | | | |
| Sat 21/03/26 | Barnoldswick Town v Glossop North End | 3-1 | | | | | | |
