| Saturday 19th August 2000 | First Division | ||
|---|---|---|---|
| Castleton Gabriels | 3-1 | Colne | |
| Tuesday 22nd August 2000 | First Division | ||
|---|---|---|---|
| Chadderton | 3-2 | Colne | |
| Saturday 26th August 2000 | First Division | ||
|---|---|---|---|
| Colne | 2-4 | Formby | |
| Monday 28th August 2000 | First Division | ||
|---|---|---|---|
| Colne | 2-3 | Padiham | |
| Saturday 9th September 2000 | First Division | ||
|---|---|---|---|
| Alsager | 1-2 | Colne | |
| Tuesday 12th September 2000 | First Division | ||
|---|---|---|---|
| Darwen | 1-0 | Colne | |
| Saturday 16th September 2000 | First Division | ||
|---|---|---|---|
| Colne | 0-5 | Squires Gate | |
| Saturday 23rd September 2000 | First Division | ||
|---|---|---|---|
| Tetley Walker | 4-0 | Colne | |
| Tuesday 26th September 2000 | First Division | ||
|---|---|---|---|
| Oldham Town | 5-2 | Colne | |
| Saturday 14th October 2000 | League Challenge Cup Preliminary Round | ||
|---|---|---|---|
| Colne | 1-2 | Castleton Gabriels | Att: 27 |
| Hayes (36) |
Leyland Mayall (4) Tom McKenna (42) | ||
| Saturday 21st October 2000 | First Division | ||
|---|---|---|---|
| Bacup Borough | 3-4 | Colne | |
| Saturday 11th November 2000 | First Division | ||
|---|---|---|---|
| Colne | 2-1 | Ashton Town | |
| Saturday 18th November 2000 | First Division | ||
|---|---|---|---|
| Squires Gate | 2-1 | Colne | |
| Thursday 30th November 2000 | First Division | ||
|---|---|---|---|
| Colne | 0-5 | Warrington Town | |
| Thursday 21st December 2000 | First Division | ||
|---|---|---|---|
| Colne | 0-2 | Daisy Hill | |
| Saturday 23rd December 2000 | First Division | ||
|---|---|---|---|
| Colne | 2-2 | Chadderton | |
| Wednesday 3rd January 2001 | First Division | ||
|---|---|---|---|
| Colne | 0-3 | Castleton Gabriels | |
| Saturday 13th January 2001 | First Division | ||
|---|---|---|---|
| Colne | 0-5 | Atherton LR | |
| Thursday 25th January 2001 | First Division | ||
|---|---|---|---|
| Colne | 0-3 | Blackpool Mechanics | |
| Saturday 27th January 2001 | First Division | ||
|---|---|---|---|
| Bootle | 2-1 | Colne | |
| Thursday 1st February 2001 | First Division | ||
|---|---|---|---|
| Colne | 0-5 | Tetley Walker | |
| Saturday 3rd February 2001 | First Division | ||
|---|---|---|---|
| Warrington Town | 4-0 | Colne | |
| Saturday 10th February 2001 | First Division | ||
|---|---|---|---|
| Stone Dominoes | 3-1 | Colne | |
| Wednesday 14th February 2001 | First Division | ||
|---|---|---|---|
| Colne | 1-5 | Nelson | |
| Saturday 17th February 2001 | First Division | ||
|---|---|---|---|
| Colne | 0-1 | Holker Old Boys | |
| Saturday 24th February 2001 | First Division | ||
|---|---|---|---|
| Padiham | 2-1 | Colne | |
| Tuesday 13th March 2001 | First Division | ||
|---|---|---|---|
| Atherton LR | 3-0 | Colne | |
| Saturday 17th March 2001 | First Division | ||
|---|---|---|---|
| Colne | 0-2 | Bacup Borough | |
| Saturday 24th March 2001 | First Division | ||
|---|---|---|---|
| Holker Old Boys | 2-1 | Colne | |
| Wednesday 28th March 2001 | First Division | ||
|---|---|---|---|
| Colne | 1-2 | Bootle | |
| Saturday 31st March 2001 | First Division | ||
|---|---|---|---|
| Colne | 2-2 | Oldham Town | |
| Tuesday 3rd April 2001 | First Division | ||
|---|---|---|---|
| Blackpool Mechanics | 2-2 | Colne | |
| Wednesday 11th April 2001 | First Division | ||
|---|---|---|---|
| Colne | 0-3 | Alsager | |
| Monday 16th April 2001 | First Division | ||
|---|---|---|---|
| Nelson | 4-0 | Colne | |
| Saturday 28th April 2001 | First Division | ||
|---|---|---|---|
| Ashton Town | 1-2 | Colne | |
| Thursday 3rd May 2001 | First Division | ||
|---|---|---|---|
| Daisy Hill | 3-2 | Colne | |
| Saturday 5th May 2001 | First Division | ||
|---|---|---|---|
| Colne | 1-3 | Darwen | |
| Wednesday 9th May 2001 | First Division | ||
|---|---|---|---|
| Colne | 1-1 | Stone Dominoes | |
| Friday 11th May 2001 | First Division | ||
|---|---|---|---|
| Formby | 2-1 | Colne | |
