| Tue 22/08/23 | Cheadle Town v Prestwich Heys * | 2-4 | | | | | | |
| Sat 26/08/23 | Prestwich Heys v Beverley Town (CUP) | 4-1 | | | | | | |
| Tue 29/08/23 | AFC Liverpool v Prestwich Heys | 2-0 | | | | | | |
| Sat 02/09/23 | Workington v Prestwich Heys (CUP) | 6-0 | | | | | | |
| Tue 05/09/23 | Prestwich Heys v Padiham | 2-0 | |  | | | | |
| Sat 09/09/23 | FC Isle of Man v Prestwich Heys | 0-2 | | | | | | |
| Tue 12/09/23 | Prestwich Heys v Ramsbottom United | 3-0 | | | | | | |
| Sat 16/09/23 | Chadderton v Prestwich Heys | 2-3 | | | | | | |
| Sat 23/09/23 | Prestwich Heys v Parkgate (CUP) | 1-2 | | | | | | |
| Sat 30/09/23 | Lower Breck v Prestwich Heys | 2-5 | | | |  | | |
| Tue 03/10/23 | Barnoldswick Town v Prestwich Heys | 3-3 | | | | | | |
| Sat 07/10/23 | Prestwich Heys v Skelmersdale United * | 2-1 | | | | | | |
| Sat 21/10/23 | Prestwich Heys v AFC Liverpool | 4-1 | | | | | | |
| Wed 01/11/23 | Litherland REMYCA v Prestwich Heys | 3-2 | | | | | | |
| Sat 04/11/23 | Colne v Prestwich Heys | 3-4 | | | | | | |
| Sat 11/11/23 | Prestwich Heys v Squires Gate | 4-2 | | | | | | |
| Tue 14/11/23 | Wythenshawe Town v Prestwich Heys | 3-1 | | | | | | |
| Sat 25/11/23 | Prestwich Heys v Litherland REMYCA | 5-2 | | | | | | |
| Tue 28/11/23 | Prestwich Heys v Cheadle Town | 2-1 | | | | | | |
| Sat 27/01/24 | Prestwich Heys v Barnoldswick Town | 3-1 | | | | | | |
| Tue 30/01/24 | Prestwich Heys v Glossop North End * | 3-2 | | | | | | |
| Sat 03/02/24 | Padiham v Prestwich Heys * | 1-0 | | | | | | |
| Sat 10/02/24 | Prestwich Heys v FC Isle of Man | 3-4 | | | | | | |
| Tue 05/03/24 | Kendal Town v Prestwich Heys | 1-0 | | | | | | |
| Sat 09/03/24 | Glossop North End v Prestwich Heys | 0-1 | | | | | | |
| Tue 12/03/24 | Ramsbottom United v Prestwich Heys * | 2-4 | | | | | | |
| Sat 16/03/24 | Prestwich Heys v Wythenshawe Town * | 0-2 | | | | | | |
| Tue 19/03/24 | Prestwich Heys v Longridge Town | 3-1 | | | | | | |
| Sat 13/04/24 | Prestwich Heys v West Didsbury & Chorlton | 3-1 | | | | | | |
| Thu 18/04/24 | Prestwich Heys v Charnock Richard | 0-6 | | | | | | |
