| Sat 02/09/23 | Route One Rovers v Atherton LR | 1-3 | | | | | | |
| Sat 09/09/23 | AFC Blackpool v Route One Rovers | 0-6 | |  | | | | |
| Mon 11/09/23 | Route One Rovers v Ashton Town (CUP) | 0-2 | | | |  | | |
| Sat 16/09/23 | Route One Rovers v Bacup Borough * | 2-4 | | | | | | |
| Sat 30/09/23 | Route One Rovers v Garstang * | 6-2 | | | | | | |
| Sat 07/10/23 | Runcorn Town v Route One Rovers * | 5-3 | | | | | | |
| Sat 14/10/23 | Atherton LR v Route One Rovers | 3-0 | | | | | | |
| Tue 24/10/23 | Knaresborough Town v Route One Rovers (CUP) | 3-2 | | | | | | |
| Sat 28/10/23 | Bacup Borough v Route One Rovers * | 5-1 | | | | | | |
| Sat 04/11/23 | Route One Rovers v Nelson | 4-1 | | | | | | |
| Sat 11/11/23 | Route One Rovers v AFC Blackpool | 2-0 | | | | | | |
| Sat 09/12/23 | Route One Rovers v Darwen FC | 3-1 | | |  | | | |
| Mon 11/12/23 | Route One Rovers v New Mills (CUP) | 2-0 | | | | | | |
| Sat 13/01/24 | Holker Old Boys v Route One Rovers | 6-1 | | | | | | |
| Sat 20/01/24 | South Liverpool v Route One Rovers | 2-1 | | | | | | |
| Sat 27/01/24 | Route One Rovers v Daisy Hill | 4-2 | | | | | | |
| Mon 29/01/24 | Route One Rovers v Steeton | 3-1 | | | | | | |
| Sat 03/02/24 | Ilkley Town v Route One Rovers | 2-1 | | | | | | |
| Wed 07/02/24 | Garstang v Route One Rovers | 1-4 | | | | | | |
| Sat 17/02/24 | Route One Rovers v Ashton Athletic | 4-2 | | | | | | |
| Sat 02/03/24 | Steeton v Route One Rovers | 1-4 | | | | | | |
| Sat 09/03/24 | FC St Helens v Route One Rovers | 5-0 | | | | | | |
| Tue 12/03/24 | Ashton Town v Route One Rovers | 5-2 | | | | | | |
| Sat 23/03/24 | Darwen FC v Route One Rovers | 3-3 | | | | | | |
| Mon 01/04/24 | Route One Rovers v Runcorn Town | 4-1 | | | | | | |
| Sat 13/04/24 | Route One Rovers v Euxton Villa | 0-3 | | | | | | |
