| Sat 26/07/25 | Darwen FC v Thornton Cleveleys * | 0-0 | |  | | | | |
| Sat 02/08/25 | Darwen FC v MSB Woolton * | 2-1 | | | | | | |
| Sat 16/08/25 | Holker Old Boys v Darwen FC * | 1-2 | | | | | | |
| Sat 30/08/25 | Darwen FC v AFC Blackpool * | 0-2 | | | | | | |
| Sat 06/09/25 | Darwen FC v Fulwood Amateurs | 1-1 | | | | | | |
| Sat 13/09/25 | Steeton v Darwen FC | 1-2 |  | | | | | |
| Sat 27/09/25 | Darwen FC v Garstang (CUP) * | 0-0 | | | | | | |
| Sat 04/10/25 | Darwen FC v Maghull | 5-2 | | | | | | |
| Sat 25/10/25 | Darwen FC v Garstang | 4-0 | | | | | | |
| Wed 29/10/25 | Darwen FC v Colne (CUP) | 4-0 | | | | | | |
| Sat 01/11/25 | Darwen FC v Ashton Athletic | 3-1 | | | | | | |
| Sat 08/11/25 | Colne v Darwen FC | 4-1 | | | | | | |
| Wed 19/11/25 | Darwen FC v Bacup Borough | 1-1 | | | | | | |
| Sat 13/12/25 | Darwen FC v Holker Old Boys | 3-3 | | | | | | |
| Fri 26/12/25 | Darwen FC v Nelson * | 0-2 | | | | | | |
| Sat 10/01/26 | Darwen FC v Droylsden * | 2-3 | | | |  | | |
| Tue 20/01/26 | Fulwood Amateurs v Darwen FC * | 2-2 | | | | | | |
| Sat 24/01/26 | Darwen FC v Maine Road * | 2-1 | | | | | | |
| Fri 30/01/26 | Barnton v Darwen FC (CUP) * | 2-2 | | | | | | |
| Wed 04/02/26 | Bacup Borough v Darwen FC * | 1-1 | | | | | | |
| Fri 27/02/26 | Darwen FC v Colne * | 0-0 | | | | | | |
| Sat 07/03/26 | Garstang v Darwen FC | 2-1 | | | | | | |
| Sat 21/03/26 | AFC Blackpool v Darwen FC * | 1-2 | | | | | | |
| Wed 25/03/26 | Darwen FC v Steeton * | 2-0 | | | | | | |
| Sat 28/03/26 | MSB Woolton v Darwen FC * | 2-1 | | | | | | |
| Sat 04/04/26 | Darwen FC v Squires Gate * | 2-1 | | | | | | |
| Mon 06/04/26 | Nelson v Darwen FC | 1-0 | | | | | | |
| Sat 18/04/26 | Droylsden v Darwen FC * | 2-2 | | | | | | |
| Thu 30/04/26 | Darwen FC v Runcorn Town (CUP) * | 2-3 | | | | | | |
