| Sat 26/07/25 | Cheadle Town v Barnoldswick Town | 2-0 | | | | | | |
| Tue 29/07/25 | Barnoldswick Town v Charnock Richard | 0-5 | | | | | | |
| Sat 02/08/25 | Trafford v Barnoldswick Town (CUP) | 2-1 |  | | | | | |
| Sat 16/08/25 | Euxton Villa v Barnoldswick Town * | 2-3 | | | | | |  |
| Sat 23/08/25 | Barnoldswick Town v Birtley Town (CUP) | 3-2 | | | |  | | |
| Mon 25/08/25 | Barnoldswick Town v Prestwich Heys | 2-2 | | | | | | |
| Sat 30/08/25 | Pilkington v Barnoldswick Town | 3-3 | | | | | | |
| Sat 01/11/25 | Barnoldswick Town v Burscough * | 1-2 | | | | | | |
| Tue 04/11/25 | Ramsbottom United v Barnoldswick Town * | 4-1 | | | | | | |
| Sat 08/11/25 | Barnoldswick Town v Winsford United (CUP) | 1-2 | | | | | | |
| Tue 11/11/25 | Barnoldswick Town v Longridge Town | - | |  | | | | |
| Sat 15/11/25 | Stockport Town v Barnoldswick Town | 2-0 | | | | | | |
| Sat 22/11/25 | Barnoldswick Town v FC St Helens | 1-1 | | | | | | |
| Sat 29/11/25 | Barnoldswick Town v City of Liverpool | 7-0 | | | | | | |
| Sat 06/12/25 | Atherton LR v Barnoldswick Town | 2-0 | | | | | | |
| Sat 13/12/25 | Barnoldswick Town v South Liverpool * | 0-0 | | | | | | |
| Sat 20/12/25 | Prestwich Heys v Barnoldswick Town * | 0-2 | | | | | | |
| Fri 26/12/25 | Padiham v Barnoldswick Town | 3-1 | | | | | | |
| Sat 17/01/26 | Barnoldswick Town v AFC Liverpool | 0-7 | | | | | | |
| Tue 20/01/26 | Bradford Park Avenue v Barnoldswick Town (CUP) | 1-3 | | | | | | |
| Sat 24/01/26 | FC St Helens v Barnoldswick Town | 5-0 | | | | | | |
| Sat 31/01/26 | Barnoldswick Town v Litherland REMYCA | 2-2 | | | | | | |
| Tue 03/02/26 | Barnoldswick Town v Longridge Town * | 2-5 | | | | | | |
| Tue 17/02/26 | Barnoldswick Town v Atherton LR * | 1-3 | | | | | | |
