| Sat 29/07/23 | Prestwich Heys v Burscough | 1-3 | | | | | | |
| Tue 01/08/23 | Prestwich Heys v Pilkington | 3-0 |  |  | | | | |
| Sat 05/08/23 | Prestwich Heys v Bootle (CUP) | 3-1 | | | | | | |
| Tue 08/08/23 | Charnock Richard v Prestwich Heys | 0-0 | | | | | | |
| Sat 12/08/23 | Squires Gate v Prestwich Heys | 2-2 | | | | | | |
| Tue 15/08/23 | Prestwich Heys v Wythenshawe | 2-5 | | | | | | |
| Sat 19/08/23 | Penistone Church v Prestwich Heys (CUP) | 0-2 | | | |  | | |
| Tue 22/08/23 | Cheadle Town v Prestwich Heys | 2-4 | | | | | | |
| Sat 26/08/23 | Prestwich Heys v Beverley Town (CUP) | 4-1 | | | | | | |
| Tue 29/08/23 | AFC Liverpool v Prestwich Heys | 2-0 | | | | | | |
| Sat 02/09/23 | Workington v Prestwich Heys (CUP) | 6-0 | | | | | | |
| Tue 05/09/23 | Prestwich Heys v Padiham | 2-0 | | | | | | |
| Sat 09/09/23 | FC Isle of Man v Prestwich Heys | 0-2 | | | | | | |
| Tue 12/09/23 | Prestwich Heys v Ramsbottom United | 3-0 | | | | | |  |
| Sat 16/09/23 | Chadderton v Prestwich Heys | 2-3 | | | | | | |
| Sat 23/09/23 | Prestwich Heys v Parkgate (CUP) | 1-2 | | | | | | |
| Sat 30/09/23 | Lower Breck v Prestwich Heys | 2-5 | | | | | | |
| Tue 03/10/23 | Barnoldswick Town v Prestwich Heys | 3-3 | | | | | | |
| Sat 07/10/23 | Prestwich Heys v Skelmersdale United | 2-1 | | | | | | |
| Tue 10/10/23 | Prestwich Heys v Kendal Town | 2-2 | | | | | | |
| Sat 14/10/23 | Longridge Town v Prestwich Heys | 2-2 | | | | | | |
| Sat 21/10/23 | Prestwich Heys v AFC Liverpool * | 4-1 | | | | | | |
| Wed 01/11/23 | Litherland REMYCA v Prestwich Heys * | 3-2 | | | | | | |
| Sat 04/11/23 | Colne v Prestwich Heys * | 3-4 | | | | | | |
| Tue 14/11/23 | Wythenshawe Town v Prestwich Heys * | 3-1 | | | | | | |
| Tue 27/02/24 | West Didsbury & Chorlton v Prestwich Heys * | 1-1 | | | | | | |
| Tue 05/03/24 | Kendal Town v Prestwich Heys * | 1-0 | | | | | | |
| Sat 09/03/24 | Glossop North End v Prestwich Heys * | 0-1 | | | | | | |
| Thu 18/04/24 | Prestwich Heys v Charnock Richard * | 0-6 | | | | | | |
