| Sat 06/08/22 | Daisy Hill v Holker Old Boys | 3-2 | | | | | |
| Sat 13/08/22 | Holker Old Boys v Garstang | 3-0 |  | | | | |
| Sat 20/08/22 | Ilkley Town v Holker Old Boys | 1-3 | | | | | |
| Sat 27/08/22 | Holker Old Boys v AFC Blackpool | 4-3 | | | | | |
| Mon 29/08/22 | Holker Old Boys v FC St Helens | 3-1 | | | | | |
| Sat 03/09/22 | Holker Old Boys v Nelson | 0-1 | | | | | |
| Sat 17/09/22 | Pilkington v Holker Old Boys | 3-2 | | | | | |
| Sat 24/09/22 | Padiham v Holker Old Boys | 0-1 | | | | | |
| Sat 08/10/22 | South Liverpool v Holker Old Boys | 1-1 | | | | | |
| Sat 15/10/22 | Holker Old Boys v Burscough | 1-1 | | | | | |
| Sat 22/10/22 | Holker Old Boys v Whickham | 3-1 | | | | | |
| Sat 29/10/22 | South Liverpool v Holker Old Boys | 1-2 | | | | | |
| Sat 05/11/22 | Runcorn Town v Holker Old Boys | 0-1 | | | | |  |
| Sat 12/11/22 | Holker Old Boys v FC Isle of Man | 3-1 | | | | | |
| Sat 19/11/22 | Darwen FC v Holker Old Boys | 0-1 | | | | | |
| Sat 26/11/22 | Holker Old Boys v Steeton | 3-3 | | | | | |
| Sat 03/12/22 | Holker Old Boys v Squires Gate | 4-2 | | | | | |
| Tue 06/12/22 | Euxton Villa v Holker Old Boys | 4-0 | | | | | |
| Mon 26/12/22 | Cleator Moor Celtic v Holker Old Boys | 2-4 | | | | | |
| Tue 17/01/23 | Bury AFC v Holker Old Boys | 4-0 | | | | | |
| Tue 24/01/23 | Holker Old Boys v Atherton LR | 2-1 | | | | | |
| Sat 28/01/23 | Garstang v Holker Old Boys | 0-6 | | | | | |
| Tue 31/01/23 | Holker Old Boys v Ilkley Town | 1-0 | | | | | |
| Sat 04/02/23 | Holker Old Boys v Bacup Borough | 3-1 | | |  | | |
| Wed 08/02/23 | Bacup Borough v Holker Old Boys | 0-0 | | | | | |
| Sat 11/02/23 | Nelson v Holker Old Boys | 2-3 | | | | | |
| Sat 18/02/23 | Ashton Town v Holker Old Boys | 0-1 | | | | | |
| Tue 21/02/23 | Holker Old Boys v Euxton Villa | 1-3 | | | | | |
| Sat 25/02/23 | Atherton LR v Holker Old Boys | 2-1 | | | | | |
| Sat 04/03/23 | Holker Old Boys v Pilkington | 0-1 | | | | | |
| Tue 07/03/23 | Holker Old Boys v Darwen FC | 2-4 | | | | | |
| Sat 11/03/23 | AFC Blackpool v Holker Old Boys | 5-1 | | | | | |
| Sat 25/03/23 | FC St Helens v Holker Old Boys | 3-1 | | | | | |
| Sat 01/04/23 | Steeton v Holker Old Boys | 1-2 | | | | | |
| Tue 04/04/23 | Holker Old Boys v Chadderton | 1-1 | | | | | |
| Sat 08/04/23 | Holker Old Boys v Daisy Hill | 1-2 | | | | | |
| Mon 10/04/23 | Holker Old Boys v South Liverpool | 2-3 | | | | | |
| Thu 13/04/23 | Holker Old Boys v Cleator Moor Celtic | 4-0 | | | | | |
| Sat 15/04/23 | Holker Old Boys v Runcorn Town | 5-1 | | | | | |
