| Sat 03/08/24 | Thornton Cleveleys v Route One Rovers | 4-1 | | | | | | |
| Tue 06/08/24 | Steeton v Route One Rovers | 1-2 | | | |  | | |
| Sat 10/08/24 | Route One Rovers v Ashton Athletic | 0-1 | | | | | | |
| Mon 12/08/24 | Route One Rovers v Holker Old Boys | 1-3 | | | | | | |
| Sat 17/08/24 | Skelmersdale United v Route One Rovers | 2-2 |  | | | | | |
| Tue 20/08/24 | Atherton LR v Route One Rovers | 4-2 | | |  | | | |
| Sat 24/08/24 | Northallerton Town v Route One Rovers (CUP) | 1-2 | | | | | | |
| Mon 26/08/24 | Route One Rovers v Maine Road | 1-1 | | | | | | |
| Sat 31/08/24 | Route One Rovers v Ashton Town | 1-3 | | | | | |  |
| Sat 07/09/24 | Daisy Hill v Route One Rovers | 1-0 | | | | | | |
| Sat 14/09/24 | Droylsden v Route One Rovers | 0-0 | |  | | | | |
| Sat 28/09/24 | Route One Rovers v Darwen FC | 3-2 | | | | | | |
| Mon 30/09/24 | Route One Rovers v AFC Blackpool (CUP) | 1-3 | | | | | | |
| Sat 05/10/24 | Route One Rovers v Atherton LR | 0-3 | | | | | | |
| Mon 07/10/24 | Route One Rovers v Ashton Athletic (CUP) | 3-0 | | | | | | |
| Sat 12/10/24 | Nelson v Route One Rovers | 5-1 | | | | | | |
| Tue 15/10/24 | Harrogate Railway Athletic v Route One Rovers (CUP) | 1-3 | | | | | | |
| Sat 26/10/24 | Maine Road v Route One Rovers | 3-1 | | | | | | |
| Sat 02/11/24 | Maghull v Route One Rovers | 3-5 | | | | | | |
| Sat 16/11/24 | Route One Rovers v AFC Blackpool | 2-3 | | | | | | |
| Mon 18/11/24 | Route One Rovers v Armthorpe Welfare (CUP) | 6-3 | | | | | | |
| Mon 02/12/24 | Route One Rovers v Atherton LR (CUP) | 1-2 | | | | | | |
| Sat 14/12/24 | Bacup Borough v Route One Rovers | 2-2 | | | | | | |
| Thu 26/12/24 | Route One Rovers v Steeton | 0-2 | | | | | | |
| Sat 28/12/24 | Route One Rovers v Nelson | 0-4 | | | | | | |
