| Sat 26/07/25 | Cheadle Town v Barnoldswick Town | 2-0 | | | | | | |
| Tue 29/07/25 | Barnoldswick Town v Charnock Richard * | 0-5 | | | | | | |
| Sat 02/08/25 | Trafford v Barnoldswick Town (CUP) * | 2-1 | | | | | | |
| Sat 09/08/25 | Litherland REMYCA v Barnoldswick Town | 1-3 | | | | | | |
| Tue 12/08/25 | Barnoldswick Town v Chadderton | 0-6 | | | | | | |
| Sat 16/08/25 | Euxton Villa v Barnoldswick Town * | 2-3 | | | | | | |
| Sat 23/08/25 | Barnoldswick Town v Birtley Town (CUP) | 3-2 | | | | | | |
| Mon 25/08/25 | Barnoldswick Town v Prestwich Heys | 2-2 | | | | | | |
| Sat 06/09/25 | Wythenshawe v Barnoldswick Town | 3-0 | | | | | | |
| Sat 13/09/25 | West Didsbury & Chorlton v Barnoldswick Town | 4-0 | | | | | | |
| Tue 16/09/25 | Longridge Town v Barnoldswick Town | 1-2 | | | | | | |
| Sat 20/09/25 | Barnoldswick Town v Sunderland West End (CUP) * | 10-1 |  | | | | | |
| Tue 23/09/25 | Barnoldswick Town v Stockport Town | 3-3 | | | | | | |
| Sat 27/09/25 | Longridge Town v Barnoldswick Town (CUP) | 1-3 | | | | | | |
| Sat 04/10/25 | FC Isle of Man v Barnoldswick Town | 1-2 | | | | | | |
| Sat 11/10/25 | AFC Blackpool v Barnoldswick Town (CUP) * | 3-0 | | | | | | |
| Tue 14/10/25 | Barnoldswick Town v Ramsbottom United | 1-3 | | | | | | |
| Sat 18/10/25 | Barnoldswick Town v AFC Liverpool (CUP) | 1-0 | |  | | | | |
| Tue 21/10/25 | Irlam v Barnoldswick Town | 1-2 | | | | | | |
| Sat 25/10/25 | Barnoldswick Town v Pilkington | 6-1 | | | | | |  |
| Tue 28/10/25 | Liversedge v Barnoldswick Town (CUP) * | 2-2 | | | | | | |
| Sat 01/11/25 | Barnoldswick Town v Burscough | 1-2 | | | | | | |
| Tue 04/11/25 | Ramsbottom United v Barnoldswick Town | 4-1 | | | | | | |
| Sat 22/11/25 | Barnoldswick Town v FC St Helens * | 1-1 | | | | | | |
| Sat 29/11/25 | Barnoldswick Town v City of Liverpool * | 7-0 | | | | | | |
| Sat 06/12/25 | Atherton LR v Barnoldswick Town * | 2-0 | | | | | | |
| Sat 13/12/25 | Barnoldswick Town v South Liverpool | 0-0 | | | | | | |
| Tue 16/12/25 | Club Thorne Colliery v Barnoldswick Town (CUP) | 0-3 | | | | | | |
| Sat 20/12/25 | Prestwich Heys v Barnoldswick Town | 0-2 | | | | | | |
| Fri 26/12/25 | Padiham v Barnoldswick Town * | 3-1 | | | | | | |
| Tue 20/01/26 | Bradford Park Avenue v Barnoldswick Town (CUP) | 1-3 | | | | | | |
| Sat 24/01/26 | FC St Helens v Barnoldswick Town | 5-0 | | | | | | |
| Sat 31/01/26 | Barnoldswick Town v Litherland REMYCA * | 2-2 | | | | | | |
| Tue 03/02/26 | Barnoldswick Town v Longridge Town | 2-5 | | | | | | |
| Sat 07/02/26 | Abbey Hey v Barnoldswick Town | 1-1 | | | | | | |
| Tue 10/02/26 | Barnoldswick Town v Irlam | 0-3 | | | | | | |
| Tue 17/02/26 | Barnoldswick Town v Atherton LR | 1-3 | | | | | | |
| Sat 21/02/26 | South Liverpool v Barnoldswick Town * | 3-1 | | | | | | |
| Tue 24/02/26 | Barnoldswick Town v Abbey Hey * | 2-0 | | | | | | |
| Tue 03/03/26 | Glossop North End v Barnoldswick Town | 1-1 | | | |  | | |
| Sat 07/03/26 | AFC Liverpool v Barnoldswick Town | 3-1 | | | | | | |
| Tue 10/03/26 | Charnock Richard v Barnoldswick Town | 3-1 | | | | | | |
| Sat 14/03/26 | Barnoldswick Town v Cheadle Town | 3-1 | | | | | | |
| Tue 17/03/26 | Chadderton v Barnoldswick Town | 3-1 | | | | | | |
