| Sat 29/07/23 | Holker Old Boys v FC St Helens | 0-1 | | | | | |  |
| Tue 01/08/23 | Holker Old Boys v AFC Blackpool | 4-1 | | | |  | | |
| Sat 05/08/23 | Euxton Villa v Holker Old Boys | 2-1 | | | | | | |
| Tue 08/08/23 | Holker Old Boys v Steeton | 0-3 | | | | | | |
| Sat 12/08/23 | Bacup Borough v Holker Old Boys | 6-1 | | | | | | |
| Mon 14/08/23 | Route One Rovers v Holker Old Boys | 4-3 | | | | | | |
| Sat 19/08/23 | Holker Old Boys v Nelson | 2-1 |  | | | | | |
| Wed 23/08/23 | Darwen FC v Holker Old Boys | 1-2 | | | | | | |
| Sat 09/09/23 | Ashton Town v Holker Old Boys * | 2-2 | | | | | | |
| Sat 16/09/23 | Holker Old Boys v Ashton Athletic | 2-1 | | | | | | |
| Sat 23/09/23 | Daisy Hill v Holker Old Boys | 2-5 | | | | | | |
| Sat 30/09/23 | Holker Old Boys v Runcorn Town | 7-1 | | | | | | |
| Sat 07/10/23 | Holker Old Boys v Atherton LR | 2-0 | |  | | | | |
| Tue 10/10/23 | Holker Old Boys v Steeton (CUP) | 3-1 | | | | | | |
| Sat 14/10/23 | Ilkley Town v Holker Old Boys | 0-2 | | | | | | |
| Tue 17/10/23 | Holker Old Boys v Daisy Hill (CUP) | 4-2 | | | | | | |
| Sat 28/10/23 | Steeton v Holker Old Boys | 0-0 | | | | | | |
| Sat 04/11/23 | Holker Old Boys v Shelley | 2-2 | | | | | | |
| Tue 21/11/23 | Ashville v Holker Old Boys (CUP) | 3-0 | | | | | | |
| Tue 28/11/23 | Holker Old Boys v Charnock Richard (CUP) | 1-2 | | | | | | |
| Sat 16/12/23 | Holker Old Boys v Euxton Villa | 2-2 | | | | | | |
| Sat 06/01/24 | Holker Old Boys v South Liverpool (CUP) | 1-4 | | | | | | |
| Sat 13/01/24 | Holker Old Boys v Route One Rovers | 6-1 | | | | | | |
| Sat 27/01/24 | Nelson v Holker Old Boys | 0-1 | | | | | | |
| Sat 03/02/24 | Garstang v Holker Old Boys | 0-2 | | | | | | |
| Sat 10/02/24 | Holker Old Boys v Darwen FC | 1-2 | | | | | | |
| Sat 09/03/24 | Holker Old Boys v Bacup Borough | 3-1 | | | | | | |
| Tue 19/03/24 | Ashton Athletic v Holker Old Boys | 2-1 | | | | | | |
| Sat 23/03/24 | Holker Old Boys v Ashton Town | 4-2 | | | | | | |
| Tue 26/03/24 | FC St Helens v Holker Old Boys | 3-1 | | | | | | |
| Sat 30/03/24 | Atherton LR v Holker Old Boys | 4-1 | | | | | | |
| Mon 01/04/24 | Shelley v Holker Old Boys | 1-2 | | | | | | |
| Wed 03/04/24 | Runcorn Town v Holker Old Boys | 0-3 | | | | | | |
| Sat 06/04/24 | AFC Blackpool v Holker Old Boys | 0-1 | | | | | | |
| Sat 13/04/24 | Holker Old Boys v Ilkley Town | 5-0 | | | | | | |
