Rylands became the first club to clinch one of our league titles this season, with their 5-0 win at Stone Alleynians ensuring they will be playing Premier Division football next season.
Tom Freeman and Andy Scarisbrick set them on the road to victory with two goals in the opening 23 minutes setting them up for a good afternoon.
Ste Wolhuter then added a third goal at the midway point of the second half and two further goals from Jay White ensured they clinched the title in fine style.
Elsewhere though, the destination of the two other divisional championship trophies are still very much in the balance.
In the Premier Division, it's City of Liverpool who have the advantage of a three point lead over Bootle with just two games left, after their 3-0 win over Padiham.
All the goals came in a 12 minute spell in the second half, with Craig Cairns, Jamie McDonald and Jack Hazlehurst on target.
Meanwhile Bootle kept up the pressure on the Purps with a 1-0 win at Squires Gate to set up a fascinating clash between the teams on Easter Monday. Victory for City of Liverpool will secure the title, with any other result meaning that the title chase will go to the final day of the season.
Carl Peers struck a vital second half winner for the Bucks to keep them on the tails of their tenants.
The defeat for Padiham at City of Liverpool also had repercussions for them at the other end of the table, as they now sit just three points above West Didsbury & Chorlton who occupy the second relegation place.
West gave themselves a lifeline with a fine 4-0 win at Litherland REMYCA, a result which combined with others elsewhere also means Remy could still go down.
Ryan Eiseet and Zachary Cooper put West two up in 28 minutes, and Jesurun Uchegbucam and Daragh Walshe added two more in the last quarter of the game.
There were also vital wins for Winsford United and Hanley Town, who both moved one point ahead of Padiham.
Sam Jenners set Winsford United on the way to a 3-0 win at Whitchurch Alport, before further strikes from Perry Bircumshaw and Steve Jenyons made the game safe, while a Neville Thompson goal on the stroke of half time saw Hanley Town to a 1-0 home win over Silsden.
Saul Henderson scored the only goal in Congleton Town's 1-0 win over Irlam to keep the Bears in third place, ten points ahead of Northwich Victoria in fourth place, who were held to a 1-1 draw at Runcorn Town.
The two goals in 1874 Northwich's 1-1 draw with Charnock Richard both came early on, with Taylor Kennerley's first minute opener being cancelled by a Carl Grimshaw penalty 15 minutes later.
The other two games in the Premier Division both ended in 3-0 away wins. Jordan Lorde, Terry Cummings and Luke Gibson scored for Burscough in the victory at Abbey Hey, and two goals in four minutes from Zack Dale added to Ethan Kershaw's opener earned Barnoldswick Town the points at Ashton Athletic.
The battle at the top of the First Division North between Longridge Town and Avro will continue into next week after both won their respective games.
For both, though, it was an uncomfortable afternoon as they had to come from behind to earn their vital victories.
Longridge Town went two goals down at home against Nelson, before eventually edging to a 3-2 win.
Gareth Hill and Dylon Allan-Meredith had put the Admirals two up, but Richie Allen pulled a goal back on the hour, Thomas Ince levelled with 15 minutes to go and Paul Turner struck the winner seven minutes from time.
Avro left it even later to secure their 2-1 win over Shelley, after an own goal gave the visitors the lead.
Reece Lyndon equalised early in the second half, and Michael Stockdale sparked big celebrations in the home camp with a 90th minute winner.
AFC Liverpool moved into third place with a comfortable 7-0 win over Atherton LR, a result which also means that LR will definitely finish in bottom place.
Anthony Lyons struck a hat trick, James McGrane scored twice and James Howell and Michael Jones were also on target.
The battle to avoid the second relegation place will continue until Eater Monday at least, with both Holker Old Boys and Daisy Hill earning what could be valuable wins.
Holker Old Boys' 3-0 over Chadderton was set up by two goals from John-Paul Stanway before Nathan Reid added a third 12 minutes from time, and two first half goals from Ashley Stott, one a penalty put Daisy Hill in the driving seat against Lower Breck.
Jack Iley added a third for Daisy again from the spot before Jamie Henders pulled one back for Breck, but an 87th minute goal from Jacob Ridings rounded off a 4-1 win for Daisy.
Prestwich Heys also secured a 4-1 home win, a Will Shawcross hat trick being the highlight of their victory over Garstang at Adie Moran Park that keeps them in fifth place.
Carlisle City are sixth, although they lost 5-4 at home to St Helens Town in a remarkable game.
David Renyard scored a hat trick for City but ended up on the losing side, with five different scorers sharing Town's goals.
Elsewhere, two goals from David Moore and one from James Sigsworth set Ashton Town on the way to a 3-2 win over AFC Blackpool, and Adrian Bellamy and Anthony Hall struck in the last 15 minutes to earn Bacup Borough a 2-1 win at Cleator Moor Celtic.
With Rylands having clinched the First Division South title with the 5-0 win at Stone Old Alleynians, attention now turns towards the runners-up spot.
Remarkably, any one of five teams could still finish in second place although Vauxhall Motors are in pole position after their 3-2 win over Ellesmere Rangers, with two successfully converted penalties from Karl Noon proving to be decisive for the Motormen.
One win from their final two games will now be enough to secure the runners-up spot for Mick McGraa's men.
Wythenshawe Town are four points behind Vauxhall in third although they had no game, ahead of FC Oswestry Town on goal difference after their 2-1 win over Cheadle Town, although Town cannot finish in second place as they only have one game remaining.
A late goal from Samuel Spridgeon earned Town the points at the Venue.
Wythenshawe Amateurs' hopes of making the runners-up spot were damaged by a 1-0 defeat at Alsager Town, Calum Downie scoring the vital goal for the Bullets, and William Buckley hit a hat trick in Sandbach United's 6-2 win over Cammell Laird 1907.
Kiarno Samms scored both goals in Stockport Town's 2-0 win over Abbey Hulton United, and United are now just two points ahead of Cheadle Heath Nomads who won 2-1 at home to Barnton.
Elsewhere, Lewis Dunn, Daniel Needham and Thomas Wakefield were on the scoresheet for Eccleshall in their 3-2 win over New Mills, and Thomas Keyworth scored twice in Maine Road's 4-0 home win over bottom club Stone Dominoes.